FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606519
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG86040.2385679931257814No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG40880.11335029705929733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA26950.036.50896532
TGGCCGT13750.036.4047670
GCGGCAC28550.034.35017833
TCGCCGG27850.033.3960148
CCGTCGC28300.032.6998145
CCCGTCG28900.032.36533744
CGGCACC31500.031.13975734
ATGGCCG12650.030.604969
GTTCCGT3800.030.0504551
GTGCCGT4800.027.394481
CCCCCGA36900.027.07434319
ACCCGCG37050.026.5221425
CGTCGCC35850.025.92187146
AGTCCGC38600.025.6022627
GCACCCC39000.025.34566936
CAGTCCG37800.025.1385676
CACCCCC39650.025.02321837
GTAGCGT2650.024.7746410
CCCCGAC40700.024.46395720
GTCCGCC40900.024.4159818
Produced by FastQC (version 0.11.5)