FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565542
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG82950.23264345224372618No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG38860.10898763778410127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14200.034.903370
CGCGGCA26550.033.80068632
GCGGCAC28400.031.60510433
TCGCCGG26900.031.3139248
CCGTCGC27300.030.94980245
CCCGTCG27450.030.8981944
TCGGATA4500.030.03882634
CGGATAG4800.028.890835
CGGCACC30850.028.87411934
GACGTAT4700.028.84378644
ATGGCCG13800.027.48687469
ACGTATC5150.027.0064245
AGGACCG5850.026.75448670
CCCCCGA34550.026.51808519
GGACGTA5150.026.31602943
CGTATCA5300.026.25061246
ACCCGCG34800.025.75281325
AGTCCGC35600.025.1461897
GTTCCGT3650.024.6661871
CGCCCTA8450.024.54112261
Produced by FastQC (version 0.11.5)