FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565542
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG109980.30845240358969267No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC72400.20305468285046147No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54870.1538896470718898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21850.061.1940844
TACGGCC21850.060.7231257
TCTACGG22950.058.1121255
CTACGGC23800.055.747916
ACGGCCA25200.052.9250268
CGGCCAT26200.051.0361489
GAACGCG26900.049.37540424
GCCATAC27250.048.8160311
GCGCGGT34450.047.64019
TGAACGC28150.047.4199723
CCATACC28700.046.4694512
CCGAACT6750.046.2951567
ATCTCGT28400.046.00874335
GCGTCTA5750.045.6662832
CCCGATC28900.045.65838631
CCTGGTT43650.045.531870
CATACCA29700.045.48521413
GCCCGAT29200.045.0586430
CGTCTAC6600.045.0198943
TGGCGCG36500.044.86849615
Produced by FastQC (version 0.11.5)