FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004133
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG147790.49195558252580696No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN138230.4601327571049617No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95810.31892729116853347No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC84670.2818450448099335No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47520.15818207782411764No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA46280.15405443101220884No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG41870.13937465485050096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG29700.061.814974
TCTACGG29700.061.814975
TACGGCC29950.060.5023427
CTACGGC30950.059.098716
ACGGCCA32650.055.7073448
CGGCCAT32700.055.414239
GCCATAC33000.054.60596511
GCGCGGT51600.052.7745639
GGGTCTA25550.052.0399252
CATACCA35000.051.9713413
CGTCTAC7050.051.6070673
GCGTCTA6400.051.0084462
CCTGGTT51050.050.71188470
CCATACC35850.050.54942312
GGCCATA36100.050.38519710
TGGCGCG55100.049.6115315
GGTCTAC30150.049.3970833
TACCACC37950.048.11058815
CCGAACT7850.047.88184767
CGCGGTG57400.047.6804510