FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090602
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG155920.5044971821023865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141340.4573219068647468No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG65510.21196517701082182No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41990.13586349843816836No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40700.13168955433278048No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC34900.11292298393646286No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG32250.10434860263469706No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC31470.10182482247795091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT16600.040.8149170
CGCGGCA46700.036.66272432
GCGGCAC47850.035.7478633
TCGCCGG48150.034.00887748
CGGCACC50400.033.8402834
CCGTCGC48650.033.16005345
CCCGTCG49100.032.81716544
GCACCCC56450.030.8326136
CCCCCGA56300.030.20672419
CACCCCC57750.029.99004237
ACCCGCG57400.029.8814525
ATGGCCG17850.029.64123569
GTCGATT2650.029.62021321
TATCGGA2350.028.9465688
GGCACCC60500.028.3354435
GTTATCG2300.028.097056
TTATCGG2450.027.7650767
CGTTCGT2000.027.2487621
AGGACCG5900.027.1747470
CCCCGAC63550.026.67032620