FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090602
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG149070.48233321534121826No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140460.4544745651494434No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC102300.3310034744040158No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC87610.2834722814519631No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50470.16330151860381892No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT49320.15958056068041113No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG41610.13463396451565102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG30800.063.372994
TACGGCC30850.062.5118987
TCTACGG31200.062.4515235
CTACGGC32200.060.4093746
ACGGCCA33600.057.4968268
CGGCCAT34250.055.909129
GCCATAC34400.055.76419411
CATACCA36700.052.36211413
GCGTCTA7800.052.316262
GCGCGGT51500.051.77789
CCATACC37700.051.0634212
GGGTCTA26050.051.040992
CGTCTAC9200.050.6379053
CCTGGTT54600.050.51233770
GGCCATA38600.050.04904610
TACCACC38750.049.49375515
GGTCTAC30500.049.3908923
CCGAACT8550.049.08070867
TGGCGCG55050.048.79920215
CGGTTCA8300.047.1153835