FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364080
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG226530.673378754369694No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG80960.24066015076930394No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70140.2084968252835842No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG59910.17808732253691945No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG52390.15573351406625288No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA48590.1444377065943735No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC36180.10754797745594635No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG36050.10716154193717152No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35460.10540771919811659No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT35370.10514018691588785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA61750.036.9465232
GCGGCAC64300.035.66206733
CGGCACC65450.034.95005434
TCGCCGG62550.034.2245448
CCGTCGC64750.033.0895745
CCCGTCG65150.033.02464344
CCCCCGA71400.032.461119
GCACCCC70350.032.44688836
CGCGCTA652.2995155E-531.89320228
CACCCCC73150.031.22553837
ACCCGCG75450.030.41426325
GGCACCC75850.030.03259535
CCCCGAC77150.029.91811420
CCGCGCG83450.027.6424527
TCGCCGT3700.027.0598451
CGTCGCC79300.027.04033946
AGTCCGC85600.027.0089867
GTTATCG2200.026.585146
CAGTCCG83450.026.5504746
CCCGACC87100.026.5502221