FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364080
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA83130.2471106513519298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69740.20730779291812323No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52870.15716035290480607No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45040.13388504435090723No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC34570.10276212218496587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29400.046.913989
TGGCGCG31200.043.87471415
CCGAACT5050.038.90741767
GTACGCA3650.038.90050565
CTACTCG35950.038.03831537
CGCGGTG36700.037.957210
CGGCATA2300.037.5624241
TACTCGG36700.037.1798138
AGTACGC4100.037.17967264
GTAGTCC37800.036.7174827
TAGTCCC38400.035.9833228
GGGGTAG16500.035.604621
CGGTATG2050.035.400291
GCCGGTT5650.035.3073653
CGGGTAG4700.035.292691
ACTCGGG40550.035.04294239
AGGATCG38950.034.8780359
TGTAGTC39550.034.81582626
GATCGCT39400.034.5382661
CGTGCCT39800.034.43836220