FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371051
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG224260.6652524687404611No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG79100.23464492231057912No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71530.21218901760904832No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58660.17401101318253567No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA48950.14520693991280464No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48910.1450882825563897No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG36680.10880879583251633No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT36330.10777054396388544No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC35040.10394384421950305No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC34340.10186734048224129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA62400.038.41013332
GCGGCAC65150.036.91008433
AGGACCG5750.036.5626170
TCGCCGG62100.036.15786748
CGGCACC67200.035.85178434
CCGTCGC64700.034.83205445
CCCGTCG65250.034.61927444
GCACCCC72800.033.17886736
CCCCCGA73300.032.83444219
ACCCGCG74100.032.31690625
GGCACCC75550.031.85826135
CACCCCC76100.031.85137737
CCCCGAC79650.030.26764520
CCGCGCG83150.028.90928827
CCTATCG605.526635E-428.6583942
CGTCGCC80650.027.92413746
CCCGACC87350.027.57158721
ACCCCCC87750.027.47758538
GTACGGC5150.027.3789114
GTACGGG10050.027.3747775