FastQCFastQC Report
Wed 8 May 2024
EGAF00005318546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413868
Total Bases255.1 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG227790.6672489973250284No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG81150.23770690606666692No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72760.2131306775774576No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT64210.18808577250204167No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA51240.15009367673266805No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50670.14842401639430697No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC38220.11195511953010487No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC37100.10867438342665856No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG36890.10805924540726237No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT35370.10360681783829954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)