FastQCFastQC Report
Wed 8 May 2024
EGAF00005318548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365861
Total Bases251.5 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG222760.6618217448670637No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG79390.2358683261132887No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70790.2103176572056897No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT63940.18996625231998587No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51140.15193734975983858No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA50590.15030329535295725No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG37900.11260120367418619No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC37600.11170990127043273No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC37120.11028381742442721No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT35030.10407441067827815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)