FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3805837
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG160800.4225088988309273No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57370.15074213635528796No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG46000.1208669735461608No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC43080.11319454826888278No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40760.10709864873351119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA47050.038.14826232
GCGGCAC50300.035.5505233
TCGCCGG47250.034.63465548
CGGCACC51750.034.4265734
CCCGTCG48450.033.74742544
CCGTCGC49350.033.06889345
ACCCGCG56150.031.81694425
CCCCCGA56900.031.68530719
GCACCCC57500.030.81286636
CCCCGAC60100.030.11875720
CACCCCC59050.030.06685337
CCGCGCG61700.029.1305327
GGCACCC63950.027.97117235
CCCGCGC65200.027.45781126
CGTCGCC61250.026.59112746
CCCCCGT62100.026.32013942
GCGCGGG62500.026.19931862
AGTCCGC69400.025.8253677
TAGTCCG3100.025.7206296
GTCGCCG64050.025.43572247
Produced by FastQC (version 0.11.5)