FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795076
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG159460.42017603863532643No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG55480.146189430725498No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG43750.11528095880029808No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC42950.11317296412509262No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41200.10856172577308071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46400.035.92875332
GCGGCAC48550.034.34266733
CGGCACC49500.033.4761434
TCGCCGG46700.033.47024548
CCCGTCG48100.032.6846344
CCGTCGC47750.032.56430445
TTCGTTG5400.032.14911331
ACCCGCG54350.031.21257225
CGTTGGA5600.031.01444233
GATTTCG4850.030.05500828
AAACGGG5600.029.16975839
CCCCCGA58950.028.75801719
GCACCCC58500.028.5108936
GTGCCGT5900.028.448941
CCCCGAC59700.028.3997320
CCGCGCG60100.028.17828227
CACCCCC59750.027.85921937
GGCACCC62150.026.72183635
CCCGCGC63850.026.57417926
CGTCGCC58900.026.52454246