FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795076
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC93100.24531788032703428No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60400.1591535979780115No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA59580.15699290343592592No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC57790.1522762653501537No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38170.10057769594074006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG12900.057.217624
TACGGCC12900.056.675887
TCTACGG13200.055.6546945
CTACGGC13900.052.601256
GCGCGGT47100.047.6697049
TGGCGCG50000.045.11275515
CCGAACT7850.043.06668567
ACGGCCA17250.042.9846158
GAACGCG17150.042.25738524
ATCTCGT17400.041.91475735
CGCGGTG54550.041.73101410
CGGCCAT18350.040.785529
GCCATAC18000.040.61610811
CCCGATC17850.040.44331731
GCCCGAT18050.039.98779730
CTGAACG18300.039.97503322
TGAACGC18250.039.70774523
TCTCGGA18500.039.299746
CGCCCGA18350.038.95120629
GCCGGTT8900.038.935873