FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793856
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG158910.418861443344186No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG56600.14918858280335365No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG45390.11964080871809579No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC43120.1136574503618482No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41020.10812218492214781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46100.038.26455732
GCGGCAC48900.035.9353133
TCGCCGG46100.035.5025848
CGGCACC51150.034.4953834
CCCGTCG47700.034.2137944
CCGTCGC48300.033.78877645
CCCCCGA55950.032.2260219
ACCCGCG56050.031.87350325
CCCCGAC58150.030.71231520
GCACCCC59050.029.88515736
CACCCCC60650.029.27323237
CCGCGCG62250.028.81801227
CGTCGCC57650.028.37659546
TTTCGCG1253.794412E-927.83791745
GTCGGTT2250.027.7333164
GGCACCC64750.027.3072735
CCCGCGC65750.027.17448226
AGTCCGC66400.027.096377
GTCGCCG60850.026.94722447
GCGCGGG61400.026.71659962
Produced by FastQC (version 0.11.5)