FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788530
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG156070.4119539768722961No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57020.15050692484947986No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG43090.11373804615510501No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC42400.11191675927074618No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40200.1061097576104716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46900.036.35536632
GCGGCAC49900.034.24424733
CGGCACC50600.033.63590634
TCGCCGG47100.032.8286748
CGTACGT751.6071808E-632.3599419
CCGTCGC48050.032.01172345
CCCGTCG48650.031.7545144
ACCCGCG54450.031.41074225
CCCCCGA57100.030.17805119
CACCCCC58100.029.1299937
GCACCCC58250.029.05228236
TCGCCGT2650.029.01721
TTCGTTG6150.028.79255731
CCCCGAC59750.028.78348220
TAGTCCG3550.028.3129866
CCGCGCG61000.028.1583927
CGTTGGA6500.027.2508433
GGCACCC63100.026.701735
GTCCGTT3650.026.5979122
CCCGCGC65050.026.40176426
Produced by FastQC (version 0.11.5)