FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788530
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89680.23671450404246502No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57650.15216983896128577No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC56360.1487648243513975No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA53630.1415588632002386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC13300.057.3150867
GTCTACG13800.054.9844674
TCTACGG14050.054.011095
GCGCGGT46300.051.1906059
CTACGGC15300.049.8248946
TGGCGCG51650.045.6162215
ACGGCCA17150.044.4484338
CGCGGTG53750.044.41768310
GAACGCG17550.042.87081524
ATCTCGT17550.041.95232435
CGGCCAT18100.041.9257359
GCCGGTT8250.041.5772933
GCCATAC18800.041.28623611
GCCCGAT18550.040.5951330
CTACTCG58200.040.58751337
CCGAACT7750.039.97826867
CCCGATC18700.039.90416331
TGAACGC19250.039.8031723
TACTCGG59800.039.44608338
CGTGCCT59750.039.2702320
Produced by FastQC (version 0.11.5)