FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122003
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG132830.42546403703007335No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107880.34554739377252364No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG43480.13926956508369787No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35490.11367702081003767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA40300.035.75031732
GCGGCAC41950.034.20066533
CGGCACC42950.033.49864234
TCGCCGG41150.033.2401248
CCGTCGC42000.032.59201445
CCCGTCG42800.032.04032544
ACCCGCG46750.030.83765825
CCCCCGA47600.030.70647619
GCACCCC49150.029.86779436
CCCCGAC49350.029.42652320
TCGCCGT3050.029.3724351
AGTCCGC53100.028.13657
GGCACCC52500.027.81618735
CCCCCGT49000.027.80613742
CACCCCC52350.027.66811837
CAGTCCG51650.027.6629416
CCGCGCG53650.026.9637227
GTCCGCC55400.026.9063828
CGCCCTA7900.026.87411761
TATCGGA1800.026.7135058
Produced by FastQC (version 0.11.5)