FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122003
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106510.34115918530507494No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC57450.1840164791641776No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43600.1396539337085839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29950.046.9922039
TGGCGCG30350.046.7144315
CGCGGTG32900.043.09163310
CTACTCG34950.040.4400637
CCGAACT5350.040.0896767
TACTCGG35000.039.8089738
CGTGCCT37850.037.71631220
GCCGGTT5750.037.0166853
GTAGTCC38650.036.787727
AGGATCG37000.036.62483659
ACTCGGG38750.036.60440439
CGGCAAT2750.036.33451
TAGTCCC39350.036.24440428
TGTAGTC40400.035.34959826
CGGATAT2050.035.2954981
GATCGCT38200.035.27409461
GTACGCA3700.034.53546565
CGGTATA1600.034.455131
CGGGTAG5250.034.1269841
GGGGGGA64200.033.596431
Produced by FastQC (version 0.11.5)