FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3129039
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN105890.3384106110534257No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC58200.1859995992379769No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44820.14323886662965848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT27150.045.6791659
TGGCGCG28350.044.23037315
CGGCATA2550.039.1990551
CTACTCG32750.038.90237
CGCGGTG31900.038.87678510
TACTCGG33600.038.24952738
CGGTATG3100.036.6918871
GTACGCA3500.036.45832465
CGTGCCT36050.035.6168720
CCGAACT5150.035.32177767
ACTCGGG37550.034.5269639
AGGATCG35050.034.4129159
GTAGTCC36850.034.2949927
TAGTCCC37150.034.13024528
TGTAGTC38100.033.42339326
GGGGTAG17250.033.1693341
CGGCATT5350.032.857481
CTTAGCG11500.032.561889
GATCGCT37000.032.5602261
GCCGGTT5650.032.226653
Produced by FastQC (version 0.11.5)