FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227470
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111140.34435641539657996No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC60050.1860590493482511No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45150.13989285725351436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT28400.047.671239
TGGCGCG29550.046.2710715
CGCGGTG32250.042.08679610
CTACTCG36700.038.23238437
TACTCGG36950.037.9946138
CGTGCCT38450.036.70939320
GTAGTCC37700.036.5508127
ACTCGGG39650.035.68428839
CCGAACT5950.035.37142667
TAGTCCC39050.035.0416128
GCCGGTT5900.034.9368063
AGGATCG38800.034.9033759
CGGGTAG5550.034.7831
TGTAGTC40550.034.39321526
CGGCAAA6250.034.196661
GCGTGCC41400.034.07504719
GGGGTAG15300.034.021821
GATCGCT39700.033.90188661
CGGTATC2350.033.7389831
GGCGCGT41750.033.65561716
Produced by FastQC (version 0.11.5)