FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200384
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140150.4379162000559933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109420.3418964724233092No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45430.1419517157941047No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37560.11736091668999721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42950.036.2898832
GCGGCAC45300.034.34819833
TCGCCGG43150.033.7271948
CGGCACC46350.033.51386634
CGTACGT1450.033.22181719
CCCGTCG45150.032.9970244
CCGTCGC44850.032.9306845
CCCCCGA50950.030.72773419
GCACCCC51200.030.64451636
ACCCGCG51400.030.13843725
CACCCCC53800.029.18435337
TACGCAC1952.0736479E-1029.06749370
CCCCGAC54350.028.56884220
GGCACCC55150.028.37055235
CCCCCGT53050.027.64681242
CGTCGCC54800.027.15819446
CTACGCA1455.140464E-927.05638169
CCGCGCG57850.026.86400227
AGTCCGC58200.026.808867
CGCGAGA4550.026.53958925
Produced by FastQC (version 0.11.5)