FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200384
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107930.3372407811062672No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC60050.18763373395192576No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44750.13982697076350836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT30400.049.6070259
TGGCGCG31900.046.95208415
CGCGGTG34950.043.14888410
TACTCGG37850.040.30227738
CTACTCG37800.040.14626737
CGTGCCT39700.038.31610520
ACTCGGG41000.037.22181739
GTAGTCC41000.036.29188527
AGGATCG41100.035.88223359
CGGCATA2600.035.8288541
TGTAGTC42000.035.7329426
TAGTCCC42350.035.4789728
GGGGTAG16000.034.717491
GATCGCT42450.034.6300861
GCGTGCC43850.034.59179719
GGGGTAC11250.034.3485151
CCGAACT5400.033.6956567
CGGTATA2050.033.660351
GGCGCGT44850.033.4717216
ATCGCTT44200.033.43991562
Produced by FastQC (version 0.11.5)