FastQCFastQC Report
Wed 8 May 2024
EGAF00005318566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388881
Total Bases253.1 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG130030.3836959751611225No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62030.18303977035487526No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43920.12960030169250558No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG42830.1263839007625231No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT37040.10929861508857938No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA36560.10788221834877058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)