FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376690
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG139360.41271185687759315No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG65550.19412501591795514No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44640.13220046850614064No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG43030.12743248565903295No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37230.11025590148932832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT21450.046.6908470
CGCGGCA44300.035.1623832
GCGGCAC45100.034.39933433
TATCGGA2100.034.3666048
AGGACCG8850.033.6596170
CGGCACC47200.033.10425634
CCGTCGC45350.032.5538645
TCGCCGG45750.032.4315748
ATGGCCG25050.032.26235669
CCCGTCG46350.031.9719844
GTTATCG2500.031.6172756
ACGATCG553.333216E-431.2423656
CGTACTA1251.3096724E-1030.2556212
TTATCGG2500.030.242617
CCCCCGA53500.029.57384919
ACCCGCG55750.028.17279625
ACCGTTA1103.7678547E-828.1490121
GCACCCC56400.028.03749736
AGTCCGC58400.027.2461287
CACCCCC58500.027.1091937
Produced by FastQC (version 0.11.5)