FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460851
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG141460.40874339866119636No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69180.1998930320895063No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45950.1327708127278522No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG43900.12684741411866618No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39310.1135847801595619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19750.048.2928670
CGTACGT2050.035.22995819
CGCGGCA45750.034.12389832
ATGGCCG22400.033.74484669
AGGACCG7050.033.45844370
GCGGCAC47400.032.87942533
CGGCACC48450.032.0358734
TCGCCGG46800.031.85031548
CCGTCGC47500.031.59321645
CCCGTCG49150.030.57701744
TACGTAG2400.030.106221
GTACGTA2650.029.8549720
GACGTAT4050.029.20510144
CCCCCGA55300.028.42089519
GCACCCC55650.028.3601436
CGATCTA2650.027.63941647
GTTCCGT4150.027.3721371
CACCCCC57650.027.3337537
TATGGCC25950.026.97171668
GTTATCG2550.026.957846
Produced by FastQC (version 0.11.5)