FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460851
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG216570.6257709447762992No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC112840.3260469751514873No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC96290.278226366867571No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG59580.17215418982209868No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT57000.16469937596273287No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA52340.1512344796120954No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45870.13253965570895712No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45360.1310660297135011No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40820.11794786889120625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG41900.063.0647664
TCTACGG42250.062.542345
TACGGCC42350.062.162047
CTACGGC42850.061.5899776
ACGGCCA46000.057.4537548
CGGCCAT46300.056.4876569
GCCATAC46800.056.1044611
GGGTCTA35550.055.5787852
CCTGGTT71700.053.75881270
GGTCTAC41850.052.712563
CCATACC50150.052.3567112
GCGCGGT53750.052.110969
CATACCA50500.052.06189713
GGCCATA51400.051.15030310
TGGCGCG55700.050.89404715
TACCACC52450.050.11689815
ATACCAC53500.049.26459514
GCGTCTA8650.048.8618352
CTGAACG53800.048.85976822
GAACGCG53850.048.70636724