FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427864
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140350.4094386475076024No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64780.1889806596761132No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44050.12850568167231838No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG43030.12553006770396957No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38280.11167304187097271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22750.049.99392770
GTTCCGT3650.037.726081
CGCGGCA44250.036.36503232
ATGGCCG25100.035.67783469
GCGGCAC46000.034.847333
TCGCCGG44950.033.92852448
CGGCACC48200.033.1299634
CCGTCGC46300.032.93010345
CCCGTCG47400.032.50855644
GACGTAT4450.031.26599944
CCCCCGA53200.030.7617419
CGTACGT2250.030.56068219
GCACCCC54000.030.24585336
AGGACCG7500.030.19241170
ACGTATC4750.029.3134145
CACCCCC55050.029.3129437
TAGTCCG2700.029.2689256
ACCCGCG56000.029.07899925
GGCACCC56850.028.22726235
CCGTATA1103.817877E-828.1108194
Produced by FastQC (version 0.11.5)