FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3670819
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96590.26312929076590263No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80110.21823467732950055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78010.21251388314160957No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71830.19567840310295878No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA65340.17799842487466694No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT42270.11515141443912108No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37000.1007949452152231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18050.059.2639964
TCTACGG18150.058.7479635
TACGGCC18950.056.0832637
CTACGGC19500.054.68086
GCGCGGT44600.051.4348229
ACGGCCA21450.050.0257458
GCCATAC22400.048.21119711
TGGCGCG48400.047.46760615
CGGCCAT22700.047.2710239
CCGAACT8050.045.2030167
CGGGTAG4950.044.727431
CATACCA24350.044.63282813
CCATACC24450.044.1689512
GCGTCTA5050.043.600482
CGCGGTG53950.042.8394710
GAACGCG24750.042.23298624
GGGTCTA20000.041.8002552
GCCGGTT8750.041.668283
CCCGATC25400.041.5507231
ATCTCGT25450.041.54783235