FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656728
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG195920.5357795274901497No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78830.2155752355657845No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69860.19104510917957257No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC54780.14980605612449163No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51470.14075424806001433No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA49050.1341363098376472No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC47040.12863959255378032No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG39890.10908659326042298No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC38340.10484783117584902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59050.038.4815332
GCGGCAC61850.036.70064533
TCGCCGG59650.035.56591848
CCCGTCG60400.035.3177644
CGGCACC64050.035.1816534
CCGTCGC60900.034.70895845
ACCCGCG67650.033.8359725
CCCCCGA68100.033.7488219
GCACCCC69600.032.52028736
GTTCCGT3650.032.3036771
CACCCCC70950.032.11675337
CCCCGAC73000.031.53804820
GGCACCC74300.030.30035635
CCGCGCG77700.029.61764527
TGGCCGT13100.029.52021870
CGTCGCC73350.028.98165946
CCCGCGC80800.028.4240926
ATGGCCG10700.028.12580969
AGTCCGC83900.027.7696637
CAGTCCG82100.027.4143836