FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656728
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95790.2619554968266713No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG79660.21784502429494346No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78500.21467278944455262No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC70480.19274061401340215No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA61250.16749946947106814No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT42360.11584126574358279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG15700.060.4837654
TACGGCC16050.058.9569057
TCTACGG16200.058.624215
CTACGGC17000.056.067846
GCGCGGT48300.050.93739
TGGCGCG50350.048.4506915
ACGGCCA20150.047.6437728
CCGAACT8250.047.5245867
CGGCCAT20850.045.8791929
GCCATAC20850.045.54912611
CGCGGTG57200.043.3726810
CTACTCG57400.043.07420737
CGTGCCT57400.042.9813520
TACTCGG57750.042.82737438
CCATACC22800.041.80267312
GTAGTCC60750.041.2218427
GGGTCTA17950.041.024022
CATACCA23600.040.67722713
TAGTCCC61800.040.6606728
GAACGCG22650.040.37337524