FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655957
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG182050.4979544343656121No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG88100.24097657603740966No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82340.22522146732032133No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC80160.21925859631281222No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC69780.19086657747889268No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT62790.17174709658784282No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT52790.14439447728734228No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC50480.13807602222892665No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA48770.13339872432854108No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44860.12270385018204537No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG43360.11860095728697029No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT41440.11334925438127419No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG38390.10500670549462152No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37930.1037484850067985No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG36820.10071234426444294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19800.041.54514370
GTTCCGT3800.041.0724641
CGCGGCA52600.038.8879532
GCGGCAC54950.037.16636733
CGGCACC56000.036.1044434
TCGCCGG53950.035.7906948
CCGTCGC54800.035.2368645
CCCGTCG54900.035.16007244
GCACCCC59600.034.22962636
CACCCCC62050.032.94532837
ATGGCCG19800.032.9229569
CCCCCGA63000.032.54269819
ACCCGCG64450.031.89740425
GGCACCC65350.031.10267335
CCCCGAC66350.030.80131520
TTCCGTT6100.030.6061922
CCCCCGT64800.029.60738642
GTGCCGT5750.029.55631
GTAGCGT4100.029.49833910
TACGGCC4500.029.17994315