FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633937
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG180030.4954131015479905No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG85190.23442894029258077No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82390.22672379845880652No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC80490.22149530935731687No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC70350.19359168857357736No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT62690.17251262198546644No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT52550.14460900120172696No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC51010.14037117319315112No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA49550.1363534920941117No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43760.12042035951641428No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG42230.11621004987153052No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT42110.11587982950722592No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG37380.10286364348088589No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT36540.10055210093075362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18550.045.51609870
CGCGGCA52600.040.13366332
GCGGCAC54550.038.773833
CGGCACC55750.037.45147734
TCGCCGG53650.036.57668348
CCGTCGC54100.036.4201945
AGGACCG5300.036.0335870
CCCGTCG55100.035.9392744
GCACCCC62500.033.59083636
CCCCCGA63100.033.42575519
ACCCGCG63600.033.1410325
GGCACCC65550.032.0710635
CACCCCC65700.031.86057537
GTGCCGT5650.031.4598881
ATGGCCG21050.031.29303769
CGTACGT2350.030.89812919
CCCCGAC68200.030.7297320
CCCCCGT64650.030.55239142
CGCCCTA11050.029.92311161
GTTCCGT4050.029.2589911