FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633937
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG189120.5204272941440647No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC173510.4774711284207734No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA154010.4238103192212743No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC137770.3791204965853839No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT90110.24796797522906974No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50460.13885766319008833No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48970.13475742699997276No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG37220.10242334966181307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG38250.063.683464
TACGGCC38600.062.8340267
TCTACGG38650.062.7561955
CTACGGC39800.060.856076
ACGGCCA42800.056.9086728
CGGCCAT43450.056.1368459
GCCATAC43300.056.01215711
GCGCGGT73550.054.535929
GGGTCTA33500.054.47332
GCCGGTT11750.054.1064383
GCGTCTA7650.053.7625852
CCGAACT12250.053.30381467
CATACCA46000.053.02418513
CCATACC46350.052.54852312
CCTGGTT65950.052.00659670
GGCCATA46950.051.87840710
CGTCTAC9050.051.5411573
TGGCGCG79100.050.75242215
CGCGGTG79650.050.48940710
TACCACC48400.050.46626715