FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3644525
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG189810.5208086101755373No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC172300.47276394043119474No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA153710.4217559215535632No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC138550.38015928001591426No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT92770.25454620286594276No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG52330.14358524087501115No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48630.13343302625170633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG37100.062.94935
GTCTACG37350.062.6204454
TACGGCC37450.062.4532367
CTACGGC38950.060.1368036
ACGGCCA40600.057.7779128
CGGCCAT41450.056.4263849
GCCATAC42100.055.80137311
GGGTCTA33150.054.5072362
CCGAACT14400.054.26343567
GCGTCTA7400.053.691082
GCCGGTT14300.053.6348343
GCGCGGT78050.053.248929
CCATACC44150.052.8191212
CGGTTCA14650.052.587845
CATACCA44750.052.1109313
CGCGGTG81750.050.71209710
TACCACC46300.050.44101315
GGCCATA46700.050.08294710
CCTGGTT67100.049.87670570
TGGCGCG83700.049.8608415