FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087415
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG162830.5273991348749683No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101360.328300536209094No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG65280.21143901937381274No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA42850.13878924601972847No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC32160.1041648110150401No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG31850.10316073478945978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49050.037.4992532
TCGCCGG48600.035.97638748
GCGGCAC51300.035.95438433
CCCGTCG50800.034.9114144
CCGTCGC50350.034.6338245
CGGCACC54050.034.53473734
GTTCCGT3550.033.084381
TGGCCGT12400.032.09564670
CCCCCGA59000.031.5619319
CACCCCC60450.031.18962137
TATCGGA2200.031.1245218
GCACCCC61250.030.6390836
ACCCGCG60950.030.53802525
CCGCGCG62800.029.5689227
GTTATCG2350.029.137856
GGCACCC65300.028.92462335
CCCCGAC65200.028.4725920
GACGTAT3450.028.333944
TTATCGG2550.028.1951547
CGTCGCC63400.027.74980546
Produced by FastQC (version 0.11.5)