FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087415
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC105980.34326451092580684No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100940.326940174871211No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89030.2883642140755292No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG83880.2716835929086307No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47790.15478968651768552No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34720.11245653726499354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG17700.060.344574
TCTACGG18050.059.5537725
TACGGCC18400.058.9752857
CTACGGC19400.055.5851336
GCGCGGT54750.049.892279
CCGAACT6750.049.53145267
GCCATAC21800.049.30462611
CGGCCAT22300.048.3526429
ACGGCCA22550.048.120188
TGGCGCG57750.047.18191515
CCATACC23300.045.98359712
GGGTCTA18050.045.718132
GCGTCTA4100.044.262982
CATACCA24250.044.18217513
CGCGGTG63550.043.25282310
CGGCAAT2550.043.2250371
GGTCTAC20400.043.128053
TACCACC25050.042.63451815
GGCCATA25950.041.94732710
CGTGCCT66400.041.231720
Produced by FastQC (version 0.11.5)