FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094346
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC107750.3482157457504752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101480.327952982633487No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC88580.2862640441631285No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG82840.26771408239414723No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT46600.15059725059834939No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34830.11256013386996801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18850.059.2075584
TCTACGG19250.058.5108035
TACGGCC19450.058.0842257
CTACGGC20500.055.108286
GCGCGGT52550.052.182359
ACGGCCA22400.050.587578
GCCATAC22400.050.28190211
CGGCCAT23000.049.267899
CCGAACT7150.047.3433667
CCATACC24100.047.16120512
TGGCGCG58350.046.99465615
CATACCA24450.046.2060613
GGGTCTA18600.046.016332
GGTCTAC20500.045.9258043
CGCGGTG61550.044.9414610
GGCCATA25350.044.70065310
CGGTTCA7750.042.407285
TACTCGG66350.041.80243738
GCCGGTT7900.041.6027573
CTACTCG66850.041.51441637
Produced by FastQC (version 0.11.5)