FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465561
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG209670.6050102710643385No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC119680.34534091305852066No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC98250.2835038829211201No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA84800.244693427701893No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61420.1772296029416305No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG56050.16173427621098No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53790.1552129655198682No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40110.11573883708871377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39780.11478661030638329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG40500.062.83915
TACGGCC40700.062.3619847
GTCTACG41400.061.4739274
CTACGGC41750.061.122676
ACGGCCA43650.058.3051648
GCCATAC44600.056.59012611
CGGCCAT45100.056.2778859
CGGTCTA5700.055.582072
GCGTCTA9050.055.1748542
GGGTCTA32600.054.8240972
CCATACC46350.054.3119812
CATACCA47100.053.4471413
CCGAACT10000.053.4158367
CCTGGTT66500.053.03526370
GGCCATA48000.052.8060210
GCGCGGT58750.052.4640779
GCCGGTT10250.051.40313
TACCACC50100.050.79514315
CGTCTAC11300.050.2837033
CTGAACG51200.049.36304522