FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449257
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG130940.3796179872940752No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG71340.20682715147059208No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC67200.19482456656607494No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA61740.17899507053258135No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51740.15000331955548687No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC51180.14837978150076958No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA50100.1452486723952434No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC49340.1430452993209842No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCC46780.13562341107084802No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38610.11193715052256181No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG35690.10347155923725022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT20000.044.41805670
ACGATCG350.001357977639.370616
CGCGGCA40800.037.045232
CCGTCGC40000.036.0389345
GCGGCAC42200.035.90916433
CCCGTCG40600.035.8288944
TCGCCGG41250.035.09251448
GTTATCG1400.034.449286
CGGCACC43750.034.3331434
GTTCCGT3450.032.2497061
ATGGCCG22200.031.80046569
CCCCCGA48850.031.18863719
CGTACGT1550.031.13400519
CACCCCC49000.030.84533137
GCACCCC49300.030.5716936
GTCGCCT2450.028.84289670
GGCACCC52200.028.78503635
ACCCGCG53400.028.69486625
GGCGATC12800.028.39222370
CGCGGAT1103.7063728E-828.1968253