FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049099
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG218540.43282969892252066No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG106960.2118397757698948No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG65020.1287754508279596No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC61840.12247729743465122No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG59920.11867463878208766No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA52390.10376108687906496No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC51490.1019785906356758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA75550.035.9308432
GCGGCAC79700.034.1122333
CGGCACC85600.031.85219234
CGTACGT3700.030.8430419
TCGCCGG76200.030.19052948
CCCGTCG76450.030.13691344
CCGTCGC77700.029.38831945
CACCCCC93950.029.3027437
CGTAGCG8150.029.2651869
CCCCCGA94300.029.04413419
GCACCCC94650.029.0045936
TAGTCCG5800.028.0110726
GTAGCGT8550.027.89605710
ACCCGCG98700.027.63277425
AGGAGGG121700.027.01304670
CCCCGAC101650.026.94751720
CGATCTA4150.026.86643847
GTTCCGT5300.026.8438851
GGCACCC102050.026.82800735
TAGCGTC8800.026.7107511