FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049099
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC138730.2747618931615324No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC121290.2402210770674134No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT68370.13541029795613038No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC52940.1048503901389139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT69650.050.4569139
CCGAACT12850.045.85451567
TGGCGCG77050.045.7885315
CGGCATA4300.042.0786671
CGCGGTG84900.041.719310
CTACTCG87350.040.8443337
CGGTTCA14200.040.3970075
TACTCGG88550.040.37765538
GCCGGTT14800.040.1598323
GTAGTCC90250.039.57775527
CGAACTT15550.039.33471768
TAGTCCC91350.038.99525528
TGTAGTC92050.038.8344926
CGTGCCT93350.038.4080320
CCTGTAG93650.038.0850124
ACTCGGG95600.037.95903439
TGCGACA15950.037.9560124
GTGGCGC93850.037.9233414
GGGGTAG23900.036.6884771
AGGATCG98350.035.1873359