FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5041781
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG220970.43827766418255765No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG109950.2180776991305255No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG65930.13076728243452065No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC60110.11922374256240008No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58840.11670479142192015No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA51390.10192826701516786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA73900.037.42605232
GCGGCAC78900.035.1913533
TCGCCGG73700.032.44226548
CGGCACC86050.032.31686434
AGGACCG10300.031.95158270
CCCGTCG77900.030.81753744
GCACCCC91150.030.60730236
CCGTCGC78200.030.5780545
TAGTCCG4750.030.5737466
CGTACGT3200.030.26668219
CACCCCC93100.030.23284137
CCCCCGA94200.029.63325719
ACCCGCG98050.028.3482725
TAGCGTC7900.028.01183311
GTAGCGT7800.027.92766210
GTACGGC9250.027.66086214
AGTCCGC102550.027.5136017
CCCCGAC102600.027.31126820
GTTCTCG25600.027.28268613
GGCACCC103200.027.02137235