FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5041781
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC135050.2678616941116641No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116850.23176333918510147No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT67540.13396059844725505No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC52620.10436788111185315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT68050.049.0272679
TGGCGCG72200.046.4435715
CCGAACT12650.041.24970667
GCCGGTT13500.040.7158053
CGGTTCA13550.040.3148355
TACTCGG83450.040.2566638
CTACTCG83750.040.22434637
CGCGGTG84050.040.064510
GTAGTCC84750.039.65648327
TAGTCCC85550.039.2926728
CGGGTAG8450.039.2058871
TGTAGTC87350.038.6276826
GGGGTAG25450.038.2296941
GTGGCGC88750.037.8217814
CGTGCCT89950.037.68067620
CGAACTT14350.037.30551568
CCTGTAG91400.037.25090424
ACTCGGG92100.037.16626439
CTTAGGC15050.035.38311418
TGCGACA16550.035.12361524