FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487577
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC163390.4684914483608533No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC118260.3390892874910002No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG64020.18356583954992248No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG58090.16656263073188063No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT53070.1521686833007558No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG51020.14629067687967895No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG50960.14611863766735472No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG50670.14528711480778775No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG47680.13671382739363175No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG46600.13361712157179612No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT46590.1335884483697421No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT45540.13057776215406855No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG43610.12504383415764012No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA43250.1240115988836949No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC39720.11388995855862107No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38200.10953163184640798No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA37850.10852806977451682No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG35950.10308016138425044No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35310.1012450764527923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT21250.047.76603770
GTTCCGT4450.038.095481
ATGGCCG23500.033.89064469
CGCGGCA21400.032.50179332
GGTAGAC10250.030.31443470
CGGCACC23550.029.84949734
GCGGCAC23800.029.0889733
CGCCCTA12750.028.25493861
GATAGGC45900.028.06182540
CATTAGC47200.027.59154113
ATAGGCA46950.027.52092641
CTGATAG47450.027.3456838
GTATGGC26600.027.2378467
GGCATTA47700.027.15786211
TCAGGAT24250.027.12152170
GCATTAG47650.027.11405612
TAGCCAC48150.026.9759916
CACGGTC13850.026.92184470
CTTACCC11550.026.90242270
TATGGCC27750.026.66503768
Produced by FastQC (version 0.11.5)