FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472307
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG223120.6425699110130527No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC221850.6389124003148339No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC191600.5517945273848194No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT99490.28652420422502967No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA91000.26207360121095286No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG62640.18039879538301193No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC58260.16778470336868256No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52100.1500443365174796No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47600.1370846529411138No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45720.1316703851358765No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA43970.12663050818951205No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC37530.10808376102689077No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37130.10693178915343601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC46350.064.0314257
TCTACGG46500.063.9025965
GTCTACG46800.063.4193884
CTACGGC47400.062.6166156
ACGGCCA50400.059.020968
CGGCCAT51200.058.1669
CCTGGTT77500.056.57172470
GCCATAC52350.056.4278711
GGGTCTA37750.056.3825722
CCATACC53650.054.9963912
GGCCATA54700.054.4442310
GCGCGGT106400.054.329069
CATACCA54400.054.11159513
GCGTCTA9300.054.0683672
GCCGGTT15400.053.6600653
CGCGGTG109200.053.03058610
CCGAACT16050.052.71653467
CGTCTAC12450.052.5466353
TGGCGCG112350.051.6356815
TACCACC57950.051.2126115