FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479109
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC163720.470580254887099No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC118960.3419266254664628No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG64510.18542103739779353No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG59940.17228549033675003No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG52430.1506995038097398No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT52170.14995218603383798No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG50830.14610062518880554No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG50680.14566948031809296No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG49510.14230655032653478No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG47270.13586812025722678No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG44930.1291422602741104No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT44900.1290560312999679No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44170.12695779292916665No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA43420.12480206857560369No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC38460.11054554485070746No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA38300.11008565698861403No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36550.10505563349696719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.10459574563487375No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35870.10310111008307013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22700.051.3430170
GTTCCGT4200.037.0468641
ATGGCCG26000.034.72694869
CGCGGCA21250.031.7515632
TCAGGAT22850.031.73704570
CGCCCTA10650.031.16927161
GTTATCG2550.031.0700346
GACGTAT2950.030.6731944
TTATCGG2700.030.6197477
CGGATAG3200.030.31448735
GCGGCAC23100.029.51850133
GATAGGC49600.029.12971340
CGGCACC23450.029.08928534
TCGGATA3600.028.8554934
TATGGCC29050.028.7500968
GTCCGTT3150.028.4375292
ATAGGCA51050.028.31588441
GGACGTA3200.028.26246543
GTATGGC29450.027.88667367
GCATTAG51300.027.59766212
Produced by FastQC (version 0.11.5)