FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479591
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC165670.47611917607557896No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC121120.3480868872232397No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG62180.17869916320625037No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG59930.17223288599148578No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG52920.1520868400912636No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT52740.15156953791408243No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG50380.14478713159104045No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG49520.14231557674450818No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG49090.14107979932124207No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG47620.13685516487426252No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT45410.1305038436988715No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT45370.1303888876594979No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG45000.1293255442952922No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA43860.12604929717314478No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC38260.10995545166084175No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA38180.10972553958209455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37800.10863345720804542No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35910.10320178434764316No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35320.10150618276688265No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG35290.10141996573735247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT21500.049.01117370
GTTCCGT3700.033.809981
CGTACGT2350.033.73514619
CGCGGCA22450.032.51421732
ATGGCCG24750.032.2561969
ACGATCG652.3461605E-531.7996986
GCGGCAC24000.030.42093733
GTCGGTT3750.030.3292354
TCAGGAT22950.030.23090670
TCCGCTA1501.8189894E-1230.1159271
GCATTAG47300.029.78797512
CTTACCC11900.029.37041370
GATAGGC48450.029.23062540
AGGACCG7150.029.18341670
CGGCACC25100.029.09994334
ATAGGCA49050.028.96062341
GTGCCGT5400.028.9576241
CATTAGC48850.028.91291413
AGTCCGC25600.028.7977737
CAGTCCG24300.028.6371776
Produced by FastQC (version 0.11.5)