FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479591
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG216110.6210787417256798No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC213170.6126294728317208No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC185710.5337121518017491No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT97700.28078012617000103No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA85140.2446839298066928No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60900.1750205699462954No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC53920.15496074107560343No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC49210.14142466743936286No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46020.1322569232993188No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC43670.1255032559861202No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40140.11535838551140062No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37720.10840354512929824No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC36580.10512729800715083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG45150.062.783135
GTCTACG45600.062.1528244
TACGGCC45850.061.598367
CTACGGC46550.060.6729436
ACGGCCA49350.057.2296838
CCTGGTT73850.056.34430770
GGGTCTA36000.056.15962
CGGCCAT50550.056.075529
GCGCGGT103000.055.7099049
GCCATAC51200.055.63270611
GCCGGTT16100.054.5431483
CCATACC52450.054.3037312
CATACCA52800.053.94376413
CGGTCTA7250.053.682022
GGCCATA53400.053.40522410
CGCGGTG107500.053.3778610
GCGTCTA8850.052.9278262
CGGTTCA16800.052.8945965
CCGAACT17050.052.2907167
CGTCTAC10900.052.129473