FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498934
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193600.5533113799803026No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC110960.31712515869118996No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA102550.29308926661663237No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC95310.27239725013389793No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG77330.22101017052622313No Hit
GGGCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGG72130.20614850122923153No Hit
GGGATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA54860.15679061108326137No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53300.15233211029416388No Hit
GGCCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGG45800.13089701034657986No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT43260.12363765649766473No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43010.12292315316607859No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT42250.12075106303805674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18500.060.198094
TCTACGG18600.059.874445
TACGGCC18600.059.507117
CTACGGC20000.055.512426
CGGCCAT20800.053.2130939
GCCATAC20800.053.21309311
ACGGCCA21500.051.798368
GCGCGGT52750.051.0965239
GCGTCTA4300.050.8559762
CCATACC22200.050.78059412
GGGTCTA16650.049.0471882
CATACCA22950.048.6745413
TGGCGCG55500.048.25695415
GGCCATA22950.048.22798510
CGCGGTG56450.047.98948310
CCGAACT6200.047.55217467
GACTCGC6050.046.866183
GAACGCG23450.046.03857824
TGAACGC23650.045.7615423
ATACGCG451.9842882E-645.57877717
Produced by FastQC (version 0.11.5)